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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Biochem Pharmacol. 2012 Dec 19;85(4):10.1016/j.bcp.2012.12.006. doi: 10.1016/j.bcp.2012.12.006

Figure 2. Pim kinase expression in ABCB1- and ABCG2- overexpressing and parental cell lines.

Figure 2

A. Pim-1, Pim-2 and Pim-3 kinase expression in cell lines expressing ABCG2 or ABCB1 and parental cells was studied by immunoblotting, and GAPDH expression was measured as a loading control. A representative blot from three separate experiments is shown. * indicates a non-specific band. In the Pim-1 immunoblot a five-fold higher quantity of protein was loaded in the HL60, HL60/VCR, 8226 and 8226/MR20 lanes. B. Pim-1, Pim-2 and Pim-3 mRNA expression, measured by RT-PCR and normalized to GAPDH expression.