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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Biochem Pharmacol. 2012 Dec 19;85(4):10.1016/j.bcp.2012.12.006. doi: 10.1016/j.bcp.2012.12.006

Figure 5. SGI-1776 decreases colony formation by ABCG2- or ABCB1-expressing, but not parental, cells in the presence of substrate, but not non-substrate, drugs.

Figure 5

A. 8226/MR20 cells were plated in methylcellulose-based medium in 24-well plates at a density of 500 cells per well with mitoxantrone or cytarabine at increasing concentrations with and without 1 μM SGI-1776 for 10 days, and colonies were counted as described in Materials and Methods. B. HL60/VCR cells were plated as above with daunorubicin or cytarabine at increasing concentrations in the presence and absence of 1 μM SGI-1776 for 10 days, and colonies were counted C. Parental HL60 cells were cultured as above with daunorubicin or cytarabine at increasing concentrations in the presence and absence of 1 μM SGI-1776 and colonies formed after 10 days were counted. Data are expressed as means ± SEM of triplicate treatments.