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. 2013 Sep 2;13(9):11507–11521. doi: 10.3390/s130911507

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Probing the differences in Ca²⁺ affinity between CH-GECO2.0, CH-GECO2.1, and R-GECO1. (A) Location of amino acid substitutions that differentiate the indicators, represented using the R-GECO1 crystal structure [8]. Mutations are labeled relative to CH-GECO2.1. (B) Ca²⁺ titration curves for variants with single mutations that revert CH-GECO2.1 CaM to CH-GECO2.0 CaM. (C) Ca²⁺ titration curves for variants with multiple mutations that revert the CaM domain of CH-GECO2.1 to that of CH-GECO2.0. (D) Ca²⁺ titrations for two of the point mutants that revert CH-GECO2.1 to R-GECO1. (E) Ca²⁺ titrations for CH-GECO2.1 variants with the CaM domain partially and completely converted to the R-GECO1 CaM domain.