Figure 2.

MNCs isolated from bone marrow display higher binding affinity to L-selectin in acidic pH. A suspension of MNCs (1 × 10⁶ cells/mL) was perfused through L-selectin coated (20 μg/mL) microtubes at a shear stress of 2.0 dyn/cm² in buffer at specified pH. Cell rolling flux was measured by counting the number of rolling cells crossing into the image frame over 1 min (unpaired t-test, error bars indicate standard error of the mean; * p < 0.05; n = 3).