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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Exp Cell Res. 2013 Jun 10;319(14):10.1016/j.yexcr.2013.05.032. doi: 10.1016/j.yexcr.2013.05.032

Figure 3. Expression of pancreatic differentiation markers in WT and RARβKO ES cells.

Figure 3

Transcript expression analyses of (A) early, (B) mid, and (C) late stage endocrine pancreatic differentiation markers in WT and RARβKO ES cells. RT-PCR analyses of (A) Nanog, Ngn3, (B) Pax6, Isl1, and (C) Ins1, Gcg, and Iapp mRNA were performed in both cell lines at 5, 11, 14, and 17 days of the differentiation protocol (Fig. 1A). Relative levels, normalized to HPRT1 levels for each marker tested, are shown in histograms (n=3; *: p≤0.05; **: p≤0.0079; ***: p≤0.0003).