Table 1.

Primer sequences used for RT-PCR

Primer	Application	Forward sequence (5′-3′)	Reverse sequence (5′-3′)	Product size (bp)
mIns1	RT-PCR	TAGTGACCAGCTATAATCAGAG	ACGCCAAGGTCTGAAGGTCC	289
mGcg	RT-PCR	CCGCCGTGCCCAAGATTTT	CCTGCGGCCGAGTTCCT	232
mSst*	RT-PCR	GAGCCCAACCAGACAGAGAA	GAAGTTCTTGCAGCCAGCTT	150
mNgn3*	RT-PCR	CTGCGCATAGCGGACCACAGCTTC	CTTCACAAGAAGTCTGAGAACACCAG	233
mRARβ	RT-PCR	GATCCTGGATTTCTACACCG	CACTGACGCCATAGTGGTA	248
mRARγ2	RT-PCR	ATGTACGACTGCATGGAATCGT	GATACAGTTTTTGTCACGGTGACAT	366
mNanog	RT-PCR	AAAGGATGAAGTGCAAGCGGTGG	CTGGCTTTGCCCTGACTTTAA	520
mRex1	RT-PCR	GAAAGCAGGATCGCCTCACTGTGC	CGATAAGACACCACAGTACACAC	641
mCyp26a1	RT-PCR	GAAACATTGCAGATGGTGCTTCAG	CGGCTGAAGGCCTGCATAATCAC	272
mPax6	RT-PCR	GCAACCCCCAGTCCCCAGTCAGA	AGTCCATTCCCGGGCTCCAGTTCA	399
mIsl1*	RT-PCR	CCCGGGGGCCACTATTTG	CGGGCACGCATCACGAA	397
mIapp*	RT-PCR	TGGGCTGTAGTTCCTGAAGC	GCACTTCCGTTTGTCCATCT	199
mPdx1	RT-PCR	CTTTCCCGTGGATGAAATCC	GTCAAGTTCAACATCACTGCC	205
mNkx6.1	RT-PCR	AGAGAGCACGCTTGGCCTATTC	GTCGTCAGAGTTCGGGTCCAG	215
HPRT1	RT-PCR	TGCTCGAGATGTGATGAAGG	TCCCCTGTTGACTGGTCATT	192

All primers for RT-PCR are designed around introns, except those marked with *.