Table 1.
Primer | Application | Forward sequence (5′-3′) | Reverse sequence (5′-3′) | Product size (bp) |
---|---|---|---|---|
| ||||
mIns1 | RT-PCR | TAGTGACCAGCTATAATCAGAG | ACGCCAAGGTCTGAAGGTCC | 289 |
mGcg | RT-PCR | CCGCCGTGCCCAAGATTTT | CCTGCGGCCGAGTTCCT | 232 |
mSst* | RT-PCR | GAGCCCAACCAGACAGAGAA | GAAGTTCTTGCAGCCAGCTT | 150 |
mNgn3* | RT-PCR | CTGCGCATAGCGGACCACAGCTTC | CTTCACAAGAAGTCTGAGAACACCAG | 233 |
mRARβ | RT-PCR | GATCCTGGATTTCTACACCG | CACTGACGCCATAGTGGTA | 248 |
mRARγ2 | RT-PCR | ATGTACGACTGCATGGAATCGT | GATACAGTTTTTGTCACGGTGACAT | 366 |
mNanog | RT-PCR | AAAGGATGAAGTGCAAGCGGTGG | CTGGCTTTGCCCTGACTTTAA | 520 |
mRex1 | RT-PCR | GAAAGCAGGATCGCCTCACTGTGC | CGATAAGACACCACAGTACACAC | 641 |
mCyp26a1 | RT-PCR | GAAACATTGCAGATGGTGCTTCAG | CGGCTGAAGGCCTGCATAATCAC | 272 |
mPax6 | RT-PCR | GCAACCCCCAGTCCCCAGTCAGA | AGTCCATTCCCGGGCTCCAGTTCA | 399 |
mIsl1* | RT-PCR | CCCGGGGGCCACTATTTG | CGGGCACGCATCACGAA | 397 |
mIapp* | RT-PCR | TGGGCTGTAGTTCCTGAAGC | GCACTTCCGTTTGTCCATCT | 199 |
mPdx1 | RT-PCR | CTTTCCCGTGGATGAAATCC | GTCAAGTTCAACATCACTGCC | 205 |
mNkx6.1 | RT-PCR | AGAGAGCACGCTTGGCCTATTC | GTCGTCAGAGTTCGGGTCCAG | 215 |
HPRT1 | RT-PCR | TGCTCGAGATGTGATGAAGG | TCCCCTGTTGACTGGTCATT | 192 |
All primers for RT-PCR are designed around introns, except those marked with *.