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. Author manuscript; available in PMC: 2014 Oct 18.
Published in final edited form as: ACS Chem Biol. 2013 Sep 9;8(10):2322–2330. doi: 10.1021/cb400541z

Figure 3.

Figure 3

The O-methyltransferases GsfB and GsfC tailor the nascent GsfA product. i.) Secondary metabolic profile of Penicillium aethiopicum showing the production of 1-4. ii.) Deletion of gsfB led to the production of 5, 7, 8, and 9. iii.) Coupled in vitro assay of GsfA and GsfC led to the production of 7 thereby confirming 9-OH regioselectivity of GsfC iv.) Deletion of gsfC led to the production of 5, 6, 16 and 17. v.) Coupled in vitro assay of GsfA and GsfB led to the production of 5, 6 and 16 thereby confirming the 3-OH regioselectivity of GsfB.