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Verifying the activity of GsfE. A.) Incubation of 18 with 10 M GsfE and 1 mM NADPH led to the production of 1. B.) Putative mechanism of GsfE showing the stereospecific 1,4-(Michael) addition of the hydride from NADPH to 18, followed by protonation of the resulting cyclohexadienolate to form 1.
