. 2013 Oct 9;14(10):20048–20063. doi: 10.3390/ijms141020048

Table 1.

PCR primers used in this study.

Name sequence	Primer sequence (5′–3′)	Usage
BdCht2-3GSP1	GAAGATTACGGCAAGGGTGGT	3′-RACE
BdCht2-3GSP2	CCATAGACACCGATGATTTCCG	3′-RACE

BdCht2-F	CTGAAGATGGCTTCTTCTTACATAA	cDNA Full-length confirmation
BdCht2-R	CAATTTTAAAACACTAAATGATATG	cDNA Full-length confirmation

BdCht2-SP1	TCCGGATCGGCTACTAGTAATGAG	Cloning 5′-flanking regions
BdCht2-SP2	GTCAAGTGTTCATAACCACCCTTGC	Cloning 5′-flanking regions

Q-BdCht2-F	ATCACACATCCGCATTTGAA	Quantitative real-time PCR
Q-BdCht2-R	CGTCGGGTACTCCCAATCTA
Q-α-Tubulin-F	CGCATTCATGGTTGATAACG
Q-α-Tubulin-R	GGGCACCAAGTTAGTCTGGA