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Embryoid body formation and qRT-PCR analysis in the absence of sialic acids. Wild-type (wt) and GNE-deficient (GNE-deficient (−/−)) embryonic stem cells were cultured in hanging drops in sialic acid free serum replacement medium for 72 h. (A) Representative micrographs were shown. Bar = 100 μm; (B) Bars represent fold change of expression of GNE-deficient embryonic stem cells compared to wild-type embryonic stem cells. Each experiment was performed twice in triplicates.
