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. 2013 Oct 15;14(10):20614–20634. doi: 10.3390/ijms141020614

Figure 6.

Figure 6

Protein level changes for sHSPs-CI and sHSPs-CII in P. ternata leaves exposed to heat stress. (A) 2-DE Western blot analysis of sHSPs-CII proteins in P. ternata leaves. The total proteins extracted from the unstressed (CK) or stressed samples at 24 h (H24) were separated by 2-DE. The portion of the gel corresponding to 12–18 kDa and pI 5.2–5.8 was excised; (B) Accumulation of sHSP-CI and sHSPs-CII proteins in the leaves of P. ternata induced by exposure to 38 °C; and (C) Accumulation of sHSP-CI and sHSP-CII proteins in the leaves of P. ternata maintained at 25 °C. Equal amounts (15 mg) of total protein were loaded onto sodium dodecylsulfate-polyacrylamide gel electrophoresis gels. The levels of sHSPs-CI, sHSPs-CII and actin proteins were estimated by immunoblotting, as described in the Materials and Methods Section.