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. 2013 Oct 15;14(10):20658–20681. doi: 10.3390/ijms141020658

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Cartilage explants treated with Il-1β did not demonstrate altered MMP-3 protein expression. Western blot analysis using antibodies to MMP-3 on cartilage explant supernatant cultured with and without IL-1β. Images of western blots for control and IL-1β treated (treatment) cartilage explants. Volumes of supernatant loaded were normalised to dry weight of cartilage. Human recombinant MMP-3 was used as a positive control. There was no difference in the relative intensity the bands following analysis in ImageJ (data not shown).