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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Dev Biol. 2013 Aug 23;383(1):10.1016/j.ydbio.2013.08.013. doi: 10.1016/j.ydbio.2013.08.013

Figure 4. WISP forms a complex with candidate target mRNAs.

Figure 4

Embryos collected at 0- to 1-hr post deposition from wild type flies were fixed and cross-linked in formaldehyde for RNA immunoprecipitation. Cytoplasmic extracts from these embryos were incubated with an immobilized polyclonal anti-WISP antibody or pre-immune serum (negative control). mRNAs enriched in the IPs were isolated and used to synthesize cDNA. RT-PCR was performed to test enrichment of mRNAs in the WISP IP vs. control IP. All data were normalized to RpL32. The average of two biological replicates and one technical replicate are shown. Error bars indicate standard deviation.