Table 2.

PAT assay validation of selected candidate target mRNAs.

Oocyte
Gene name	Transcript ID	Rank	Score(d)	PAT assay
CG4880	CG4880-RA	6	4.76	+
CG11971	CG11971-RA	23	4.15	+
Klp10A	CG1453-RE	38	4.02	+
CG8180	CG8180-RA	39	4.01	+
klp67A	CG10923-RA	40	4.01	+
CDC6	CG5971-RA	83	3.82	+
sced	CG3273-RA	103	3.76	+
mei-S332	CG5303-RA	170	3.59	+
dmos*	CG8767-RA	260	3.45	+
geminin	CG3183-RA	369	3.31	+
AurB	CG6620-RA	540	3.16	+
sse	CG10583-RA	709	3.05	+
cyclin B*	CG3510-RA	1083	2.84	+
cyclin A	CG5940-RA	1247	2.77	+
HSF	CG5748-RD	1557	2.66	+
sce	CG5595-RA	1896	2.56	+
psq	CG2368-RI	2968	2.28	−
mr	CG3060-RA	3225	2.21	−
bifocal	CG1822-RB	3899	2.06	+
eIF4G	CG10811-RB	4013	2.03	+
CG5316	CG5316-RB	4077	2.02	−
Embryo
Gene name	Transcript ID	Rank	Score(d)	PAT assay
pimples	CG5052-RA	19	4.18	+
punt	CG7904-RA	197	3.76	+
spinster	CG8428-RB	300	3.67	−
CG8485	CG8485-RD	492	3.55	+
costa	CG1708-RA	600	3.48	−
bj1	CG10480-RA	737	3.43	+
CG5262	CG5262-RA	787	3.40	+
CG2921	CG2921-RA	998	3.33	+
CG33298	CG33298-RB	1087	3.30	−
Pp1alpha-96A	CG6593-RA	1376	3.21	+
ubcE2h	CG2257-RA	1569	3.16	+
tango11	CG30404-RB	1674	3.13	+
Dsor1	CG15793-RA	2146	3.03	+
CG8180	CG8180-RA	2164	3.03	+
bicoid*	CG1034-RE	2293	3.00	+
grapes	CG17161-RA	2346	2.99	+
string	CG1395-RA	2477	2.96	+
Toll*	CG5490-RA	3313	2.78	+
CG10209	CG10209-RA	3738	2.69	+
cyclin A	CG5940-RA	3767	2.68	+
mei-p26	CG12218-RA	5702	2.29	+
CG8370	CG8730-RA	6476	2.13	+
CG34398	CG34398-RC	6551	2.11	−

^*previously identified WISP target

⁺Shift in electrophoretic mobility due to WISP

⁻No shift in electrophoretic mobility due to WISP