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Published in final edited form as: Methods Cell Biol. 2009 Dec 4;93:10.1016/S0091-679X(08)93002-8. doi: 10.1016/S0091-679X(08)93002-8

Fig. 3 — (a) Cartoon illustration depicting sedimentation assays. Cells with wild type motility remain relatively evenly suspended (on left), where as motility mutants sediment (on right). Degree of sedimentation is quantitated using OD600 (arrow). (b) Example dataset derived from a sedimentation assay (adapted from Ralston et al. 2006). Depletion of RSP3 by Tet-inducible RNAi (RSP3 + Tet) cause cells to sediment, while uninduced cells, expressing wt level of RSP3 (RSP3-Tet) remain suspended over time. Adapted from Ralston et al. 2006, with permission. (c) Illustration depicting a motility chamber. (d) Example dataset derived from a motility trace assay (adapted from Hutchings et al 2002). Open circles represent cell position at 1 second intervals, arrowheads indicate ending position. Uninduced cells, expressing wt level of Trypanin (-Tet) exhibit robust motility, whereas Trypain depleted cells (+Tet) fail to exhibit productive forward translocation. Respecitive western blots (WB) against Trypanin or tubulin as a loading control show protein levels. Adapted from Hutchings et al. 2002. JBC, with permission.

Fig. 3 — (a) Cartoon illustration depicting sedimentation assays. Cells with wild type motility remain relatively evenly suspended (on left), where as motility mutants sediment (on right). Degree of sedimentation is quantitated using OD600 (arrow). (b) Example dataset derived from a sedimentation assay (adapted from Ralston et al. 2006). Depletion of RSP3 by Tet-inducible RNAi (RSP3 + Tet) cause cells to sediment, while uninduced cells, expressing wt level of RSP3 (RSP3-Tet) remain suspended over time. Adapted from Ralston et al. 2006, with permission. (c) Illustration depicting a motility chamber. (d) Example dataset derived from a motility trace assay (adapted from Hutchings et al 2002). Open circles represent cell position at 1 second intervals, arrowheads indicate ending position. Uninduced cells, expressing wt level of Trypanin (-Tet) exhibit robust motility, whereas Trypain depleted cells (+Tet) fail to exhibit productive forward translocation. Respecitive western blots (WB) against Trypanin or tubulin as a loading control show protein levels. Adapted from Hutchings et al. 2002. JBC, with permission.