Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Nov 9.

Published in final edited form as: Methods Cell Biol. 2009 Dec 4;93:10.1016/S0091-679X(08)93002-8. doi: 10.1016/S0091-679X(08)93002-8

Fig. 5 — (a) Illustration of two vectors (pLEW100 and pLEW82), commonly used for inducible expression in T. brucei. Adapted from Wirtz et al. 1999, MBP, with permission. (b) Example of a PCF cell line expressing a GFP-tagged flagellum protein (PFR2) through the use of pLEW100 (Fig. 5a). Top panels, fluorescence image of a Tet induced, live cell. On bottom, series of images showing the movement of the GFP-PFR2 expressing cell line under fluorescence microscopy (see supplementary movie 2. Cells are co-stained with a live DNA dye, CFSE to visualize nucleus and kinetoplast. Movie is recorded using a 63× objective (recorded and played back at 30fps).

Fig. 5 — (a) Illustration of two vectors (pLEW100 and pLEW82), commonly used for inducible expression in T. brucei. Adapted from Wirtz et al. 1999, MBP, with permission. (b) Example of a PCF cell line expressing a GFP-tagged flagellum protein (PFR2) through the use of pLEW100 (Fig. 5a). Top panels, fluorescence image of a Tet induced, live cell. On bottom, series of images showing the movement of the GFP-PFR2 expressing cell line under fluorescence microscopy (see supplementary movie 2. Cells are co-stained with a live DNA dye, CFSE to visualize nucleus and kinetoplast. Movie is recorded using a 63× objective (recorded and played back at 30fps).