Skip to main content
. Author manuscript; available in PMC: 2013 Nov 9.
Published in final edited form as: Methods Cell Biol. 2009 Dec 4;93:10.1016/S0091-679X(08)93002-8. doi: 10.1016/S0091-679X(08)93002-8

Fig. 7. Combination of RNAi and heterologous expression for structure-function studies.

Fig. 7

In the example, knock-down of the target gene is achieved by RNAi against the gene’s 3′UTR. A tagged copy of the target gene, WT or carrying directed point mutations is expressed simultaneously from a different vector.