. Author manuscript; available in PMC: 2013 Nov 9.

Published in final edited form as: Methods Cell Biol. 2009 Dec 4;93:10.1016/S0091-679X(08)93002-8. doi: 10.1016/S0091-679X(08)93002-8

Table 1.

Most commonly used epitope tags for in situ tagging of T. brucei genes

Tag	Tag sequence (* C-terminal stop)	Antibody (vendor)	Antibody specificity	Selected references
HA	YPYDVPDYA*	HA.11 monoclonal mouse (Convance)	Highly specific in WB and IF	Oberholzer et al. FASEB, 2007
3xHA	YPYDVPDYAYPYDVPDYAYPYDVPDYA*	HAY-11 polyclonal rabbit (Santa Cruz)	Background in WB and IF. Needs pre- absorbtion	Siegel et al. Genes and Development, 2009 Zamudio et al. 2009, Mol.Cell.Biol.
3xHA	YPYDVPDYAYPYDVPDYAYPYDVPDYA*	HA monoclonal mouse 12CA5 (Roche)	Background in WB and IF	Umeyama et. al, 2008, Eucary. Cell

c-Myc	EQKLISEEDL*	Anti-c-Myc monoclonal mouse	Highly specific in WB and IF	Zhao et al. EMBO, 2008
3xc-Myc	GGRSRSEEQKLISEEDLLRSEQKLISEDLLRSEEQKLISEEDLL*	9E10 (Santa Cruz Biotechnology)		Oberholzer et al. FASEB, 2007 Kunz et al. 2009

TY1	EVHTNQDPLD*	BB2, monoclonal mouse (Prof. K. Gull, Oxford)	Highly specific in WB and IF	Bastin et al. 1996 Portman et al. JBC, 2008 Lowell et al. 2004

PTP	ProtC-TEV-ProtA-ProtA			Schimanski et al. Eucaryotic cell, 2005 Zamudio et al. 2009, Mol.Cell.Biol. Nguyen et al. 2007, MBP