Table 2.

Concentrations of antibiotics most commonly used for selection of PCF and BSF trypanosomes.

Antibiotic	PCF Final concentration	BSF Final concentration
G418	15μg/ml [1,2,3]	2.5μg/ml [1] 1–3μg/ml [2] 15μg/ml [3]
Phleomycin	2.5μg/ml [1,2,3]	2.5μg/ml [1] 1–2.5μg/ml [2] 5μg/ml [3]
Hygromycin	25–50μg/ml [1,2,3]	5μg/ml [1] 4–5μg/ml [2] 50μg/ml [3]
Puromycin	1μg/ml [2,3]	0.1μg/ml [2,3]
Blasticidin	10μg/ml [2]	5μg/ml [2]

References

^[1]Wirtz et al., 1999. MBP

^[2]Homepage of Prof. G. Cross (http://tryps.rockefeller.edu/trypsru2)

^[3]Hill lab

Note: Concentration may vary dependent on the expression level of the antibiotic resistance marker, e.g. plasmid versus in situ expression, choice of promoter and UTR, etc…