Table 3.

Most commonly used vectors¹

Name	Description and application	Antibiotic resistance marker2	Reference
pLEW100 (Fig. 5a)	All purpose inducible expression using divergent promoters	Phleo	Wirtz et al. 1999
pLEW82 (Fig. 5a)	All purpose inducible expression using a single promoter	G418	Wirtz et al. 1999
pKR10 (Fig. 7)	pLEW100 modified to include HA tag and MCS for N-terminal or C-terminal tagging	Puro	Ralston and Hill, unpublished.
pZJM (Fig. 6b)	All purpose RNAi vector with opposing, Tet- inducible promoters	Phleo	Wang et al. 2000
p2T7 (Fig. 6a)	All purpose RNAi vector with opposing, Tet- inducible promoters	Hygro	LaCount et al. 2002
pMOTag series (Fig. 4, suppl. Fig. 1, suppl. Table 1)	Series of vectors for in situ tagging with epitope, fluorescent protein (See suppl. Table 1 for complete list of pMOTag variations and related vector systems)	G418, Puro, Hygro, Phleo, Blast	Oberholzer et al. MBP, 2006 http://www.izb.unibe.ch/res/seebeck/index.php
pC-PTP-Neo, pN-PTP-Puro	Plasmids for in situ tagging with PTP tandem affinity purification tag	G418, Puro	Schimanski et al. 2005

¹Many additional plasmids and variations exist. Plasmids listed are those commonly used and for which the authors’ laboratory has direct experience.

²For most plasmids, several different antibiotic resistance markers are available.

Abbreviations: Phleo: phleomycin resistance, G418 : G418 resistance, Puro : Puromycin resistance, Hygro : Hygromycin resistance, Blast: Blasticidin resistance