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. 2013 Nov 5;6:1563–1572. doi: 10.2147/OTT.S52973

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© 2013 Fang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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McA-RH7777 cells after CoCl₂ treatment exhibited the properties of enhanced invasion and epithelial–mesenchymal transition.

Notes: (A) CoCl₂-treated McA-RH7777 cells showed a significant increase in cell invasion capacity compared with McA-RH7777 control cells. *P<0.05. (B) CoCl₂-treated McA-RH7777 cells were morphologically distinct from the typical epithelial appearance of McA-RH7777 cells, showing a spindle shape with less cell–cell adhesion. (C) E-cadherin expression was reduced, but the expression of N-cadherin and vimentin was increased in CoCl₂-treated McA-RH7777 cells relative to McA-RH7777 cells; this was examined by immunofluorescence.

Abbreviation: CoCl₂, cobalt chloride.