Comparison of experimental and simulated mouse sperm distributions within the channels of space-constrained microfluidic sorting (SCMS) microchips after varying incubation times. (A) Distribution of sperm within the microchannel after incubation for 1 h. Experimental results are compared with the computational model with different parameters: (1) Persistent Random Walk (PRW), (2) PRW and initially 25% of sperm are dead, (3) PRW including 30 minutes average exhaustion time of sperm (± 15 minutes), and (4) PRW including both exhaustion time and initially 25% dead sperm. (B) Distribution of sperm within the microchannel after an incubation period of 5 min, 15 min, 30 min and 1 h. Experimental results are compared with simulation results from PRW model with both exhaustion time and 25% initially dead sperm population for the corresponding incubation time. Data were presented as average ± standard error.