Figure 3.
Evaluation of channel length and incubation time by using space-constrained microfluidic sorting (SCMS) systems for mouse sperm sorting. The effective sorting of the microchannels with varying channel lengths was illustrated through (A) Curvilinear velocity (VCL), (B) Straight-line velocity (VSL), (C) Linearity of sperm (LIN), and (D) percentage of motile sperm at the inlet and the outlet of each channel for 30 min and 1 h of incubation. The statistical significance between channel lengths were marked with *, and between inlets and outlets were marked with #. Data were presented as average ± standard error (SEM) (N=22–109).