Table 2. PRIMERS FOR REAL-TIME RT-PCR.

			Primer set I						Primer set II
Accession nr	Target Array (nt)		Forward(5’-3’)	Nucleotide Position (nt)	Reverse (5’-3’)	Nucleotide Position (nt)	Product (bp)		Forward(5’-3’)	Nucleotide Position (nt)	Reverse (5’-3’)	Nucleotide Position (nt)	Product (bp)
AF260922	P1311-1375		Agaacaagccaccctacacg	1214	Cccactggatgatgactcact	1342	129		Aggctgtgtctgttggactg	66	Aagtggatgttgccttggtt	165	100
NM_020083	3625-3689		agagcacggtacctgcagac	3604	tggtagggagctggagagaa	3698	95		tgccttctgaatctgatgactc	3184	agtcaatggaatccccatca	3290	107
NM_012530	1102-1166		cttccgaggtcgaacaggt	1100	tacttctgcgcagggatcat	1214	115		atctggcacaacgacaacaa	718	ggcggaaaacttccttcata	823	106
U05675	1097-1161		accgaggccaacaagtacc	1093	tgtggatggtcatggttctg	1207	115		acggaatactgccacactcc	664	acctcccttccgaatgatct	753	90
U05675	1097-1161		cgaaatggaggactggaaag	1032	gccagcagtccctttgtact	1146	115		tgctacagggtgtgagttgc	324	aggtgaccgaagaggtctca	439	116
AF053990	655-719		ccactgttgacaggagcatc	608	tcacttggagctcacagttct	712	105		cggcttatatccatgcatcc	121	tccttggaggcttcaacatc	226	106
D00688	1460-1524		aaggatgttccagccattga	1441	ccacagaagtggaaacacca	1540	100		accagagcttccacctgaga	858	tcatgcagccacaatagtcc	979	122
J00793	347-411		ggaactggctgagtgtttcc	351	tcctcgtctgtcacatgctc	461	111		gctgcctttgacatgtttga	133	ttcctctttggtgggtgtct	234	102
AF091565	278-342		ctggttcaaacatcccttgc	280	acaggccagtttcaggacag	378	99		cccaggaagaccatctcctt	73	catgctcaggagcacacact	162	90
M75148	2123-2187		catcttgaggacaggcgtta	2089	cagcacatgcctcactccta	2184	96		cagccctaggagcttgtcag	1904	tggcaaagctacatgtatccag	2003	100
PBGD	–		–	–	–	–	–		atgtccggtaacggcggc	1	caaggttttcagcatcgctacca	135	135

Primer set I contained the sequence of the oligonucleotide as spotted on the microarray; Primer set II contained a fragment of the gene which was not present on the array; target array, position oligonucleotide fragment as spotted on the microarray; PBGD, house keeping gene porphobilinogen deaminase;