Table 3. DIFFERENTIALLY EXPRESSED GENES 6 HOURS AFTER MECHANICAL LOADING.
| Accession nr | Gene name | M | A | FDR (%) | B | Biological connection |
|---|---|---|---|---|---|---|
| AF260922 | matrix extracellular phosphoglycoprotein (MEPE) osteoregulin; osteoblast/osteocyte factor 45 (OF45) | 0.87 | 11.60 | 5.4 | 2.35 | skeletal development; regulation of bone remodeling; negative regulation of bone mineralization; |
| NM_020083 | GTPase activating RANGAP domain-like 1 (garnl1); tuberin-like protein 1 (tulip 1); GAP-related interacting protein to E12 (GRIPE)[47] | 0.51 | 11.61 | 5.4 | 1.92 | regulation of transcription |
| NM_012530 | creatine kinase (muscle form) (CKM) | -1.39 | 9.24 | 6.5 | 1.44 | phosphocreatine biosynthesis and metabolism |
| U05675 | fibrinogen B beta chain | -1.18 | 10.18 | 7.4 | 1.08 | blood coagulation; wound healing; regulation of blood pressure; positive regulation of cell proliferation |
| AF053990 | tissue-type vomeronasal neurons putative pheromone receptor V2R2B | 0.68 | 7.84 | 9.4 | 0.56 | related to Ca2+-sensing receptor and metabotropic glutamate receptors |
| D00688 | monoamine oxidase A | -2.00 | 8.75 | 9.4 | 0.50 | behavior; catecholamine catabolism and metabolism; dopamine catabolism; electron transport; neurotransmitter catabolism |
| J00793 | troponin-C | -1.96 | 7.64 | 9.4 | 0.42 | Ca2+-binding-subunit of the troponin complex |
| AF091565 | QFG-TN1 olfactory receptor | 0.55 | 7.64 | 14.5 | -0.06 | G-protein coupled receptor protein signaling pathway; perception of smell, sensory transduction of chemical stimulus |
| M75148 | kinesin light chain C | -1.06 | 7.88 | 16.0 | -0.25 | – |
Nine genes were found to exhibit a significant differential gene expression (FDR < 20%) in loaded tibia compared to contra lateral control tibia measured with microarray analysis. This table shows their gene IDs, gene names and their biological connection (PubMed nucleotide; Rat Genome Database).
M 2,log (LOAD/control); A positive value of M represents an up-regulation and a negative value of M represents a down-regulation in the loaded tibia compared to the non-loaded contra lateral control tibia; A, average log intensity (2log ((LOAD)*(control))0 5 ; FDR, false discovery rate; B, posterior odds; B, natural log (chance that a gene is differentially expressed)/(chance that a gene is not differentially expressed);