Figure 5. Effect of L. longipalpis saliva on the C3b deposition of classical and alternative complement pathways of dogs and guinea pigs at pHs of 7.4 and 8.15.

The guinea pig alternative pathway could not be assessed at a pH of 8.15 because the alkalinisation itself affects the pathway's efficiency in a way that the difference between the positive and negative controls is undetectable. The results are expressed as the mean of percentage of C3b deposition ± SD. Three replicates were performed per experiment.