Figure 6.

Effect of 5-HTTLPR genotype on NMDA receptor subunit mRNA expression. The genotype was simplified to a comparison of SS homozygotes with LS heterozygotes plus LL homozygotes. Although the four-factor interaction term (transcript × area × group × genotype) was significant on the 2^−ΔC_T scale (F_4,100 = 2.599, P = 0.041), this finding must be treated with caution as it did not achieve significance on the ΔC_T scale (F_4,100 = 1.275, P= 0.29). Newman–Keuls testing showed there was =a difference in mean mRNA expression averaged across the three transcripts: in SFC, expression in SS cirrhotic alcoholics was significantly lower than that in combined controls plus non-co-morbid alcoholics, P = 0.023; in PMC, expression in LL LS cirrhotic alcoholics was significantly lower than that in combined controls plus non-co-morbid alcoholics, P = 0.014. For the transcripts considered separately, no pair-wise comparison reached significance on this test, but NR1 expression in SS cirrhotic alcoholics was lower by Fisher LSD test than that in SS controls (P = 0.033), SS uncomplicated alcoholics (P = 0.036), and combined SS controls plus non-co-morbid alcoholics (P = 0.019). NR1 expression in LL + LS cirrhotic alcoholics was also lower than that in LL + LS controls on this criterion (P = 0.023). Key as for + Figure 5.