Table 3.
Effects of LC (0.5-5 mM) on LVEDP, LVDP, HR, RPP and CFR in the control and treated isolated rat hearts during pre-reperfusion and 120 min reperfusion in protocol 2. Data are expressed as Mean±SEM
| Group | Factor | Pre-Rep | Reperfusion |
||||||
|---|---|---|---|---|---|---|---|---|---|
| 5 min | 15 min | 30 min | 45 min | 60 min | 90 min | 120 min | |||
| Control | LVEDP | 8.9±1.8 | 18.1±2.8*** | 14.7±2.0** | 13.4±2.0* | 11.9±1.8 | 10.4±2.0 | 6.5±1.0 | 5.5±0.9 |
| LVDP | 100±16 | 117±16 | 115±13 | 108±10 | 104±14 | 100±10 | 90±12 | 80±13 | |
| H R | 224±15 | 240±23 | 224±16 | 219±17 | 244±17 | 220±17 | 227±16 | 190±13 | |
| RPP | 22319±1904 | 27697±2870 | 25224±1816 | 23384±1836 | 25525±2277 | 21250±1910 | 20619±2032 | 15054±1133* | |
| CFR | 4.9±0.6 | 7.4±0.5* | 6.6±0.5 | 5.6±0.5 | 5.1±0.5 | 4.6±0.5 | 4.2±0.5 | 3.9±0.7 | |
| LC (0.5 mM) |
LVEDP | 2.5±0.7 | 6.3±0.7* | 7.3±2.3* | 6.1±1.6* | 5.3±1.9 | 4.5±1.4 | 3.5±0.9 | 3.0±0.9 |
| LVDP | 112±12 | 125±9 | 121±9 | 117±8 | 114±10 | 111±16 | 112±9 | 110±8 | |
| H R | 177±16 | 184±42 | 201±22 | 185±23 | 179±21 | 179±15 | 140±20 | 114±15 ** | |
| RPP | 19880±1916 | 23027±5334 | 24260±2729 | 21619±2656 | 20445±2427 | 19873±1641 | 15472±2028 | 12340±1468** | |
| CFR | 2.1±0.4 | 4.4±0.9 | 3.9±0.8 | 3.3±0.8 | 3.1±0.9 | 2.8±0.8 | 2.5±0.8 | 2.3±0.8 | |
| LC (2.5 mM) |
LVEDP | 4.5±0.7 | 9.2±2.4 * | 8.5±0.9 * | 8.0±1.0 * | 5.8±0.8 | 4.8±1.2 | 3.5±1.2 | 3.3±1.4 |
| LVDP | 116±14 | 131±12 | 136±15 | 132±14 | 130±16 | 127±15 | 119±15 | 115±19 | |
| H R | 198±16 | 227±17 | 212±10 | 205±10 | 216±15 | 221±13 | 189±21 | 178±13 | |
| RPP | 23328±2801 | 29856±2570* | 29490±1339* | 27578±1071 | 28264±3056 | 27997±2221 | 22410±2539 | 20362±1804 | |
| CFR | 3.3±0.3 | 6.1±0.5* | 5.8±0.6* | 5.1±0.5 | 5.0±0.5 | 4.6±0.5 | 4.0±0.5 | 3.3±0.4 | |
| LC (5 mM) |
LVEDP | 5.8±1.9 | 11.5±1.0** | 10.0±2.0* | 10.8±2.2* | 9.5±1.0* | 8.0±1.4 | 5.3±1.3 | 4.4±0.7 |
| LVDP | 122±12 | 145±14 | 144±13 | 140±13 | 138±12 | 134±10 | 128±15 | 127±15 | |
| H R | 245±20 | 289±5 | 273±28 | 256±13 | 242±11 | 249±8 | 231±17 | 197±25 | |
| RPP | 30160±2736 | 41801±1367** | 39496±5102** | 35960±2463 | 33418±2341 | 33348±2493 | 29709±2713 | 24601±2996 | |
| CFR | 3.2±0.3 | 6.9±0.5** | 5.9±0.4* | 5.2±0.5* | 4.5±0.4 | 4.2±0.6 | 3.7±0.5 | 3.5±0.5 | |
*** P<0.001, ** P<0.01, *P<0.05 versus pre-reperfusion value by using repeated ANOVA with LSD post test. n=6-8 in each group. LC; L-carnitine, LVEDP; left ventricular end diastolic pressure (mmHg), LVDP; left ventricular developed pressure (mmHg), HR; heart rate (beats/min), RPP; rate pressure product (mmHg.beats.min-1), CFR; coronary flow rate (ml/min), Pre-Rep; Pre-reperfusion time