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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Jul 11;33(9):10.1161/ATVBAHA.113.301637. doi: 10.1161/ATVBAHA.113.301637

Table 1.

Disaccharide analysis of hepatocyte heparan sulfate

Strain Disaccharides (mole %)
D0A0 D0S0 D0A6 D0S6 D2A0 D2S0 D2A6 D2S6
Cre − Ndst1f/f
(Wildtype) 		40.1 16.5 15.9 9.6 0.8 6.3 0.1 10.7
Cre+Ndst1f/f
(HSPG receptor-deficient) 		63.3 3.9 17.8 11.6 0.1 1.5 0.0 1.9
Cre+Lrp1f/fLdlr−/−
(HSPG receptor-only) 		43.8 18.7 14.7 7.9 0.7 6.5 0.0 7.7
Cre+Ndst1f/fLrp1f/fLdlr−/−
(Triple receptor-deficient) 		65.1 3.2 16.1 13.9 0.1 0.6 0.1 1.1

Heparan sulfate purified from isolated hepatocytes was digested with heparin lyases I, II, and III. The resulting disaccharides were derivatized with isotopically labeled aniline and quantified by mass spectrometry (see methods). Disaccharides are designated using the code established in 40. Those containing the letter S contain a N-sulfate group.