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. Author manuscript; available in PMC: 2014 Jul 22.
Published in final edited form as: Chembiochem. 2013 Jul 3;14(11):10.1002/cbic.201300234. doi: 10.1002/cbic.201300234

Figure 5.

Figure 5

Western blot analysis of the transfer of Nedd8 variants from phage selection to NAE, Ubc12 and cullin 3. Nedd8 variants were expressed with an N-terminal GST fusion. The formation of Nedd8 conjugates was detected by Western blot with an anti-GST antibody.