Skip to main content
. Author manuscript; available in PMC: 2014 Jul 22.
Published in final edited form as: Chembiochem. 2013 Jul 3;14(11):10.1002/cbic.201300234. doi: 10.1002/cbic.201300234

Figure 6.

Figure 6

Peptide transfer through the Nedd8 enzymatic cascade and inhibition of Nedd8 transfer through the cascade by selected peptides. A) Loading of biotin-labelled peptides on NAE, Ubc12 and cullin 3. The Western blot was probed with a streptavidin-HRP conjugate. B) Inhibiting Nedd8 loading on NAE by Nedd8-mimicking peptides. NAE, Ubc12 and cullin 3-Rbx1 were first incubated with the Nedd8-mimicking peptides followed by the addition of HA-Nedd8. Attachment of HA-Nedd8 to cascade enzymes and cullin 3 was probed with an anti-HA antibody.