Fig. 2.

FoxO1, but not FoxO3a, protein level increases within ischemic mouse muscle, corresponding with an increase in THBS1 protein expression. FoxO1, FoxO3a and THBS1 (a, b, c) protein levels were assessed in murine gastrocnemius muscle 2, 4 or 7 days following femoral artery ligation and corresponding sham (Sh) operations and quantified relative to αβ-tubulin or β-actin. Data are mean ± SEM; n = 4–6. One-way ANOVA shows a main effect of arterial ligation on the levels of FoxO1 and THBS1 but not on FoxO3a (p = 0.02, p = 0.015 and p > 0.05, respectively, one way ANOVA). * indicates differences versus Sham (Bonferroni post hoc analysis, p < 0.05)