Fig. 5.

Ischemia-dependent increases in FoxO1 protein do not occur in FoxOΔ mice. FoxO1 protein levels were assessed at 4 and 10 days post-femoral artery ligation in gastrocnemius muscles of FoxO^L/L and FoxOΔ mice. Protein levels were expressed relative to β-actin. Vertical line delineates two different blots. Data are mean ± SEM. Two-way ANOVA indicates a main effect of FoxO deletion (p < 0.0001, n = 4–18 per group). Significant differences between FoxO^L/L and FoxOΔ are *p < 0.05 and **p < 0.01 (vs. time matched group), and #p < 0.05 (vs. corresponding sham, Bonferroni post hoc)