Fig. 5.

BLI correlates with increased β cell mass induced by high-fat feeding. a MIP-Luc-VU mice fed a high-fat diet (black squares) displayed a progressive increase in body weight as compared with MIP-Luc-VU mice fed a regular diet (open circles). b Over the same time period MIP-Luc-VU mice fed the high-fat diet (black squares) had higher bioluminescence emission than MIP-Luc-VU mice fed a regular diet (open circles). c Light emission from a luminescent bead implanted at the pancreas was higher for mice fed a regular diet (left) than for a mouse fed a high-fat diet (right). d Correlation between the animal weight and the amount of light detected from a luminescent bead placed at the site of the pancreas. e Immunocytochemistry of an islet stained for insulin from mice fed a regular diet (left) or high-fat diet (right). f Morphometric analysis of insulin labeling of islets revealed greater β cell mass in mice fed the high-fat diet (white bars), reflected by increased BLI measurements (black bars), but more closely correlating with BLI measurements normalized for animal body habitus (hatched bars; unpaired t test; *p<0.05; **p<0.01; ***p<0.005).