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. Author manuscript; available in PMC: 2014 Jul 2.
Published in final edited form as: Circulation. 2013 May 24;128(1):10.1161/CIRCULATIONAHA.112.001268. doi: 10.1161/CIRCULATIONAHA.112.001268

Figure 3.

Figure 3

Titin I-band segment extension. A) Antibodies were used to demarcate the tandem Ig, N2B and PEVK segments of titin and B) labeled skinned fibers from 6 WT and IG KO cardiac sarcomeres were studied using immunoelectron microscopy. Z-disk to epitope distances were calculated for WT (C) and KO (D) sarcomeres across a range of SLs. Black lines indicate the lengths of N2B and Proximal Ig segments. E) Segment extension estimated via monoexponential fits indicate that the proximal Ig segment is shortened by nearly 30nm at 2.3 μm and that the extension of the remaining I-band segments is increased with N2B segment extension dominating (F).