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. Author manuscript; available in PMC: 2013 Nov 11.
Published in final edited form as: Phytopathology. 2012 Jul;102(7):10.1094/PHYTO-02-12-0022-R. doi: 10.1094/PHYTO-02-12-0022-R

Fig. 9.

Fig. 9

Visualization of superoxide anions (O2−) by nitroblue tetrazolium (NBT)-staining 12 h postinoculation (hpi) (B and F), 24 hpi (C and G), and 48 hpi (D and H) with Pseudomonas syringae pv. tomato. No staining was observed on the controls at the time of inoculation (A) and 48 h after injection of a 10 mM MgCl2 solution without bacteria (E). First O2− staining in form of large stained areas was observed 12 hpi (B) in the avirulent infection and remained similar at 24 and 48 hpi (C and D). No staining could be observed 12 hpi on leaves inoculated with the virulent strain (F). First small spots of O2− accumulation (circles) were observed 24 hpi (G), which increased in size 48 hpi (H) on plants inoculated with the virulent strain of Pseudomonas syringae pv. tomato. Bars = 0.5cm.