Figure 3.

Proximal tubule-specific expression of AT_1aR/GFP in a representative Agtr1a^−/− mouse kidney 2 week after intrarenal adenoviral transfer. (A) AT_1aR/GFP expression (green) in proximal tubules (PT). (B) Alexa Fluor 594-labeled megalin expression (red) in proximal tubules. (C) DAPI-stained nuclei (blue) in the same kidney section. (D) Merged image of (A–C), showing the colocalization of AT_1aR/GFP and megalin expression (yellow) in proximal tubules. Only very low levels of AT_1aR/GFP and megalin expression are visible in the glomerulus (G) and cortical collecting tubules (CCT). (E) AT_1aR/GFP expression in the outer medulla. (F) Alexa Fluor 594-labeled megalin expression in the outer medulla. (G) DAPI-stained nuclei in the outer medulla. (H) Merged image of (E–G), showing the lack of AT_1aR/GFP and megalin expression in the outer medulla. Magnification: ×40. Reproduced from Li and Zhuo with permission (42).