Figure 4.

Effects of proximal tubule-specific, adenovirus-mediated transfer of ECFP/ANG II on ECFP/ANG II expression in the renal outer cortex and freshly isolated proximal tubule of mouse kidneys 2 wk after gene transfer. (A) ECFP expression (blue-green). (B) DAPI-stained nuclei (red). (C) Merged image of (A,B), respectively, in the outer renal cortex of a representative rat transferred with ECFP/ANG II selectively in proximal tubules. (D–F) Expression of ECFP/ANG II in a freshly isolated representative proximal convoluted tubule. Bars = 100 μm for the renal cortex and 10 μm for the isolated proximal tubule. G, glomerulus; PT, proximal tubule. Reproduced from Li et al. with permission (77).