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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nat Methods. 2013 Jun 30;10(8):741–743. doi: 10.1038/nmeth.2532

Figure 3.

Figure 3

Heritable, targeted gene disruptions in genes that lead to no obvious phenotypes. (A) A table summarizing the results of the three experiments, in which 93 disruptions were found out of 173 mCherry-positive F1 animals. (B) Sequences of the indel mutations found in several of our mutant lines. Insertions are marked in blue, deletions are marked by dashes, and the PAM is marked in purple. (C) Sequence at the klp-12 locus showing the target PAM site in purple and the MfeI restriction site in green. (D) An image of a 1% agarose gel showing a restriction digest of PCR amplicons spanning the klp-12 cleavage site from seven F1 animals. Wild type sequences in lanes 1 and 2 are cut into bands of 280bp and 107bp, while doubly disrupted sequences remain full length at 387bp in lanes 6 and 7. Lanes 3, 4, and 5 show all three bands, indicating worms that are singly disrupted.