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. 2013 Jun 25;22(22):2944–2953. doi: 10.1089/scd.2013.0243

FIG. 3.

FIG. 3.

Expression of GABAergic transmission output and reuptake/termination components. RT-PCR analysis of GABA synthesis enzymes GAD65 and GAD67, vesicular transporter VIAAT, cell membrane transporters GAT1, GAT2, GAT3, and BGT1, and degradation enzyme GABA-T of ES (A) and ECS (B) cells. (C) Western blot analysis of GAD67 and GAD65 of ES and ESSCs. (D) Immunofluorescence staining analysis of GAD67, GAD65, GABA-T, and VIAAT of ES cells. DAPI represents the cell nucleus position. MB, mouse brain, as a positive control for GABA synthesis enzyme. Scale bar: 20 μm. GAD, glutamic acid decarboxylase; VIAAT, vesicular inhibitory amino acid transporter.