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Comparison between experimentally determined hot spot residues (A) and predicted hot spot residues (B and C). Results of (B) and (C) are generated by using PredHS-SVM and PredHS-Ensemble respectively. The green surface represents nidogen-1 (PDB code 1GL4, chain A), while the purple surface denotes perlecan IG3 (PDB code 1GL4, chain B). Residues shown in red are either experimentally determined or predicted as hot spots.
