FIG. 1.

Expression of extrinsic apoptotic pathway components in human embryonic stem cells (hESC) and human-induced pluripotent stem cells (hiPSC). (A) Representative histograms of death receptor expression in hESC (line CCTL14) and hiPSC (clone 4) as determined by flow cytometry. Solid line, specific antibody; dashed line, isotype control. (B) Quantification of levels of expression of death receptors in hESC (CCTL 14) and hiPSC (CCTL12) as determined by flow cytometry. Medians of fluorescent intensity were normalized to the isotype control. Each bar represents the mean of n>3 experiments. Error bars show standard error of mean (SEM). (C) Western blot analysis of the expression of intracellular components of the extrinsic apoptotic pathway and selected regulators of apoptosis in two hESC cell lines (CCTL12, CCTL14) and one hiPSC line (clone 4). Alpha tubulin was used as a loading control.