FIG. 4.

Inhibition of protein synthesis in hESC and hiPSC rapidly reduces levels of endogenous cFLIP and Mcl-1. The hESC (CCTL 14) and hiPSC (clone 4) cells were left untreated (control) or treated with 50 nM HHT for 1.5 and 4 h. (A) The expression of TRAIL receptors in untreated (control, solid line) and HHT-treated (HHT, dashed line) hESC and hiPSC after 4 h of treatment was compared by flow cytometry. Representative histograms of three independent experiments are shown. (B) Western blot analysis of the expression of extrinsic apoptotic pathway components in control and HHT-treated cells (1.5 and 4 h). Alpha tubulin was used as a loading control.