FIG. 2.

Recovery of reprogramming efficiency with overexpression of FoxO3a-GFP in the wild-type and null MEFs. (A) The merged pictures of light and GFP in the FoxO3a-wild type and -null MEFs with overexpression of either GFP or FoxO3a-GFP. (B) The total cell numbers of FoxO3a-wild type and -null MEFs after 5–7 days infection of either GFP or FoxO3a-GFP retroviruses. (C) Overview of AP staining in the control group (without GFP or FoxO3a-GFP infection), GFP group, and FoxO3a-GFP group with the overexpression of Yamanaka factors for 15–20 days. (D) The number of AP+ colonies was normalized to the control group of FoxO3a-wild type MEFs. *p<0.05. Scale bar=50 μm. Color images available online at www.liebertpub.com/scd