FIG. 4.

Teratoma formation assay of FoxO3a-wild type and -null iPS cells. (A) FoxO3a-wild type and -null iPS cells were subcutaneously injected into the dorsal flank of severe combined immunodeficiency mice and teratomas formed after 5 weeks. (B) Weight of teratomas derived from FoxO3a-wild type and -null iPS cells. (C) Hematoxylin and eosin stain staining of paraffin sections of teratoma derived from FoxO3a-wild type (upper panels) and -null iPS cells (lower panels). (D) Immunohistochemistry staining for Tuj, α-SMA, and AFP was carried out on paraffin sections of teratomas derived from FoxO3a-wild type (upper panels) and -null (lower panels) iPS cells. Scale bar=50 μm. α-SMA, alpha smooth muscle actin. Color images available online at www.liebertpub.com/scd