FIG. 6.

Conversion of iPS cells into neuronal lineages in adherent monoculture. (A) FoxO3a-wild type and -null iPS cells were plated onto 0.1% gelatin-coated tissue culture plastic in Dulbecco's modified Eagle's medium/F12 with N2 and B27. At 0, 6, and 12 days, protein samples were collected for FoxO3a, Nestin (neural stem cell marker), Tuj (neuronal lineage marker), GFAP (astrocyte marker), and Oct3/4 (pluripotency marker). (B) At 6 days, differentiated cells were fixed with 4% paraformaldehyde and stained with Nestin (red), Sox2 (green), and DAPI (blue). Upper panels were from FoxO3a-wild type iPS cells and lower panels were from FoxO3a-null iPS cells. (C) Tuj (red), Oct3/4 (green), and DAPI (blue) were examined through immunofluorescence staining at 6 days. Upper panels were from FoxO3a-wild type iPS cells and lower panels were from FoxO3a-null iPS cells. Scale bar=50 μm. Color images available online at www.liebertpub.com/scd