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. Author manuscript; available in PMC: 2014 Aug 16.
Published in final edited form as: Circ Res. 2013 Jun 25;113(5):10.1161/CIRCRESAHA.113.301202. doi: 10.1161/CIRCRESAHA.113.301202

Figure 1. In vitro characterization of stem cells.

Figure 1

A) CBSC or CDC lysates were analyzed by Western analysis. Positive controls include mouse endothelial fibroblasts (MEF), liver, bone marrow (BM), and B lymphocytes (B Cell). Myocyte (MYO) lysates were used as negative controls for all samples. B) CBSCs (green) were fixed in vitro and immunostained against each paracrine factor (red). Nuclei are labeled with DAPI (blue) and scale bars = 20 μm. C) CBSCs or CDCs were allowed to proliferate over 72 hours and their culture media was analyzed by ELISA for the presence of soluble HGF, IGF, SCF, SDF-1, and VEGF. Samples were analyzed in triplicate and background signal was subtracted using unconditioned media blanks. NS = No Significant difference (p > 0.05).