Fig 1.

Expression of NPY and its receptor subtypes on hESCs and MEF feeder cells. (A) Semi-quantitative RT-PCR analysis of mRNA expression for NPY, NPY1R, NPY2R, NPY4R and NPY5R in hESCs (H9 and HES-7) and MEFs. GAPDH was used as a loading control. (B) Immunofluorescent analysis of NPY protein expression in H9 cells and MEFs. Cell nuclei were counterstained with DAPI. Bar π 50 μm. (C) Real-time qRT-PCR analysis of mRNA expression of NPY, NPY1R and NPY5R in undifferentiated and differentiated H9 hESCs (RA-differentiated hESCs and differentiating hEBs). The results are displayed as the relative mRNA level with the level in undifferentiated hESCs cultured in MEF-CM referred set to 1 and are presented as the mean ± S.E. (n= 3).