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Metformin inhibits colony formation of ovarian cancer cells. 2000 cells/well (A2780, CP70, C200, SKOV3ip, PE01 and PE04) in 6-well plates were treated with indicated concentrations of metformin, every third day for 2–3 weeks until colonies were formed. The colonies were stained with MTT and counted. The data represent three separate experiments done in triplicates. ***P < 0.001; **P < 0.01, *P < 0.05; NS: not significant compared to untreated cells.
