Fig 6.

Metformin inhibits lipid biosynthesis in ovarian cancer cells. (A) A2780, CP70, C200 and SKOV3ip cells were treated with indicated concentrations of metformin. After 12 hrs treatment, cells were pulsed with 1 mCi of [¹⁴C] acetate for an additional 4 hrs. Incorporation of labelled acetate into non-polar lipids was examined by HPTLC followed by 3 days exposure on X-ray film at –708C. Densitometric analysis was performed on the films and represented as bar graphs. ***P < 0.001; **P < 0.01, *P < 0.05; NS: not significant, compared to untreated cells.